Composite

Part:BBa_K2666005

Designed by: Léa Meneu   Group: iGEM18_Montpellier   (2018-09-29)


ScFV YLP20 with signal peptid YncM with a L. jensenii specific strong promoter

The Montpellier iGEM team 2018 designed a construction that produce and secrete YLP20, a nanobody against YLP12 in Lactobacillus jensenii (more information). For that, we used the promoter RpsU, a L.jensenii specific strong promoter [1]. The signal peptid is B. subtilis specific [2]. Figure 1 illustrates the detailed design.

Figure 1 : Construct design. The sequence that encode scFv YLP20 with a SP YncM and produce by a strong promoter RpsU.

YLP20 is This ASA targets the YLP12 antigen that is present on spermatozoa. It inhibits sperm zona binding and motility of spermatozoa [3]

Proof of function Test anti-microbien ?

Sequence and feature seq on benchling


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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